8th TURKISH-US
CYTOMETRY WORKSHOP
September 17- 20, 2024
Acıbadem University
İSTANBUL/TÜRKİYE
Theoretical Sessions
• The Basics of Flow Cytometry Instrumentation
• The Basics of Fluorescence
• Spread and Multicolor Panel Design
• Quality Control and Troubleshooting
• Applications of Flow Cytometry
• Basics of Data Analysis
• Spectral Flow Cytometry
• Exosomes and Extracellular Vesicles
• High Dimensional Data Analysis
• Experimental Design
• How to Assess Your Data
• Flow Cytometry in Cellular Immunotherapies
Focus on Hematology
• Clinical Cytometry
• Immunophenotyping in Leukemia & Lymphoma
• MRD Analysis in Acute Leukemias
• Diagnosis and MRD Analysis in Myeloma
• Standardization of CD34 and PNH analysis
Practical Sessions
• Data Analysis with Real-life Cases
• Leukemia and Lymphoma
• MRD Analysis in ALL & AML & Myeloma
• Basic Multicolor Flow Cytometry
• Cell Preparation and Phenotyping
• Panel Design
• Advanced Analysis
ORGANIZING COMMITTEE
Esin CETIN AKTAS
Istanbul University
Aziz Sancar Institute of Experimental Medicine
Department of Immunology
Klara DALVA
Ankara University
Hematology Department Laboratory
Gunnur DENIZ
Istanbul University
Aziz Sancar Institute of Experimental Medicine
Department of Immunology
Batu ERMAN
Acibadem University
Department of Molecular Biology and
Genetics
İlknur KOZANOGLU
Acibadem Labmed
Clinical Laboratories
Awtar KRISHAN
ISAC Educational Committee
Zosia MACIOROWSKI
ISAC Educational Committee
Ali SENGUL
Medical Park Antalya Hospital
Tolga SUTLU
Acibadem University
Department of Molecular Biology and
Genetics
DAY 1 (September 17)
INTRODUCTION TO FLOW CYTOMETRY
DAY 1 – INTRODUCTION TO FLOW CYTOMETRY | ||
08:30 – 09:00 |
| |
09:00 – 09:15 | Welcome Speeches by the Host and ISAC Delegates | |
| Vice Rector of Acıbadem University | Güldal Süyen |
President of Turkish Society of Immunology | İhsan Gürsel | |
Members of ISAC Education Committee and ISAC Live Education Delivery Task Force | Zosia Maciorowski | |
Günnur Deniz | ||
Awtar Krishan | ||
09:15 – 10:45 | Session 1. Moderators: İhsan Gürsel, Günnur Deniz | |
09:15 – 10:00 | The Basics of Flow Cytometry Instrumentation |
|
10:00 – 10:45 | The Basics of Fluorescence | Zosia Maciorowski |
10:45 – 11:15 | Coffee Break | |
11:15 – 12:45 | Session 2. Moderators: Batu Erman, İlknur Kozanoğlu | |
11:15 – 12:00 | Spread and Multicolor Panel Design | Maria Jaimes |
12:00 – 12:45 | Quality Control and Troubleshooting | Zosia Maciorowski |
12:45 – 14:00 | Lunch Break | |
14:00 – 15:30 | Session 3. Moderators: Raif Yücel, Esin Çetin Aktaş | |
14:00 – 14:30 | Applications of Flow Cytometry | Günnur Deniz |
14:30 – 15:00 | Basics of Data Analysis | Maria Jaimes |
15:00 – 15:30 | Experimental Design and How to Assess Your Data | Maria Jaimes |
15:30 – 16:00 | Coffee Break | |
16:00 – 18:00 | Session 4. Moderators: Tolga Sütlü, Arzu Aral | |
16:00 – 16:30 | Exosomes and Extracellular Vesicles | John Nolan |
16:30 – 18:00 | Lab 1: Panel Design |
|
Main room | Maria Jaimes Zosia Maciorowski Raif Yücel | |
DAY 2 (September 18)
LABORATORY PRACTICALS
DAY 2 – LABORATORY PRACTICALS | ||
09:00 – 10:30 | Lab 2. Basic Multicolor Flow Cytometry | |
Main room (with Cytoflex) | Zosia Maciorowski | |
10:30 – 11:00 | Coffee Break | |
11:00 – 11:30 | Session 5. Moderators: Raif Yücel, Esin Çetin Aktaş | |
11:00 – 11:30 | Spectral Flow Cytometry | Maria Jaimes |
11:30 – 13:00 | Lab 3. Cell Preparation and Phenotyping | |
Group A: Cytoflex, conventional cytometer | Esin Çetin Aktaş | |
Group B: Aurora, spectral cytometer | Maria Jaimes | |
Group C: Canto II, conventional cytometer | Raif Yücel | |
13:00 – 14:00 | Lunch Break | |
14:00 – 15:30 | Lab 4: Flow Cytometry Applications | |
Group A: Imaging Flow Cytometry (Amnis Flowsight) | Owen Hughes | |
Group B: EV Analysis (Cytoflex) | John Nolan | |
Group C: Spectral Flow Cytometry (Aurora) | Maria Jaimes | |
15:30 – 16:00 | Coffee Break | |
16:00-17:30 | Lab 5: Flow Cytometry Applications | |
Group A: Imaging Flow Cytometry (Amnis Flowsight) | Owen Hughes | |
Group B: EV Analysis (Cytoflex) | John Nolan | |
Group C: Spectral Flow Cytometry (Aurora) | Maria Jaimes | |
DAY 3 (September 19)
FOCUS ON HEMATOLOGY
DAY 3 – FOCUS ON HEMATOLOGY | ||
09:00 – 10:30 | Session 6. Moderators: Klara Dalva, Güneş Esendağlı | |
09:00 – 09:30 | Clinical Cytometry Introduction | Paul Wallace |
09:30 – 10:00 | Immunophenotyping in Leukemia | Maria Arroz |
10:00 – 10:30 | Immunophenotyping in Lymphoma | Maria Arroz |
10:30 – 11:00 | Coffee Break | |
11:00 – 12:30 | Session 7. Moderators: Güher Saruhan Direskeneli, Barbaros Oral | |
11:00 – 11:30 | MRD Analysis in Acute Leukemias | Klara Dalva |
11:30 – 12:00 | Diagnosis and MRD Analysis in Myeloma | İlknur Kozanoğlu |
12:00 – 12:30 | Standardization and Enumeration of CD34 and PNH cells | Paul Wallace |
12:30 – 13:30 | Lunch Break | |
13:30 – 14:00 | Satellite Symposium (Beckman Coulter) FLEXible Flow Solutions: Analysis and Sorting of cells…and everything smaller than cells. Dr. Andreas Wicovsky, Sr. Global Market Development Manager Research Flow Cytometry | |
14:00 – 15:00 | Session 8. Moderators: Arzu Aral, İlknur Kozanoğlu | |
14:00 – 14:30 | CSF Immunophenotyping | Maria Arroz |
14:30 – 15:00 | Flow Cytometry in Cellular Immunotherapies | Tolga Sütlü |
15:00 – 15:30 | Coffee Break | |
15:30 – 16:00 | Satellite Symposium (Becton Dickinson)
Title TBA
| |
16:00 – 17:45 | Data Analysis with Real Cases | |
16:00 – 16:45 | Leukemia and Lymphoma | Maria Arroz |
16:45 – 17:30 | MRD analysis in ALL & AML & Myeloma | Klara Dalva İlknur Kozanoğlu |
17:30 – 18:15 | CD34 & PNH | Paul Wallace |
DAY 4 (September 20)
FUTURE OF FLOW CYTOMETRY
DAY 4 – ADVANCED FLOW CYTOMETRY | ||
09:00 – 11:00 |
Session 9. Moderators: Barbaros Oral, Zosia Maciorowski
| |
09:00 – 09:30 | Imaging Flow Cytometry | Owen Hughes |
09:30 – 11:00 | Data Analysis Using Flowjo (Practical) | Raif Yücel |
11:00 – 11:30 | Coffee Break | |
11:30-12:30
| Session 10. Moderators: Günnur Deniz, Tolga Sütlü | |
11:30-12:15 | Closing Conference Tracking a Lifetime of Adventures in Flow Cytometry while Fostering Everlasting Friendships | Paul Wallace |
12:15-12:30 | Closing Ceremony | |
REGISTRATION
Registration Fee for Participation in Scientific Sessions ……………………………….………150 Euro
Participation in Scientific Sessions + Laboratory Sessions*………………………………….300 Euro
VAT (20%) is not included in the registration fees.
Registration fee includes:
participant name badge, participation certificate, meeting materials, entrance to the meeting
center, participation in scientific meetings, coffee breaks and lunches.
*Practical sessions registration is limited to 45 pax.
For your registration request, please send e-mail to istanbul1@devent.com.tr
FOLLOW THIS LINK FOR REGISTRATION